CSR Biotech Cell Xtreme

Whole Life-Cycle Super-Resolution Microscope

The Cell Xtreme is a state-of-the-art super-resolution microscope developed by CSR Biotech for long-term live-cell imaging. Integrating the MI-SIM and MI-ODT modalities, the Cell Xtreme combines a fluorescence super-resolution technology with a label-free super-resolution technique – achieving 1 + 1 > 2!

With the MI-ODT module, observe the morphology and dynamics of live cells and organelles through differences in refractive index in your sample. Combine that with super-resolution imaging of specifically fluorescently labeled organelles, proteins, and labels to get the complete understanding of your sample and its context.

The Cell Xtreme integrates numerous imaging modes, reconstruction modes, and advanced features, enabling you to create highly customized experimental workflows that perfectly address your specific research objectives.

MI-ODT Features

• Label-Free SR Preview: Preview your ODT image using 10 Hz real-time ODT reconstruction
• Real-Time Virtual Staining: Segment multiple organelles live and post-reconstruction
• 130 nm Label-Free Super-Resolution: Investigate the finest structures in your sample with excellent resolution
• Extremely Low Phototoxicity: Observe your live cells for weeks without phototoxic effects
• Multi-Site Tracking: Boost your throughput by tracking cells in multiple FOVs simultaneously
• Event-Driven Imaging: Image only when needed, with customized event-driven control

MI-SIM Features

• Real-Time SIM SR Preview: Preview your SIM image using 24 Hz real-time SIM reconstruction
• 60 nm Ultra-High Resolution: Investigate the smallest structures in your sample with extraordinary detail
• 1500 fps Ultra-Fast Recording: Capture every movement in your sample
• Ultra-Low Photobleaching: Image up to multiple days without phototoxic effects to your sample
• Multi-Site Tracking: Boost your throughput by tracking cells in multiple FOVs simultaneously
• Event-Driven Imaging: Image only when needed, with custom event-driven control

Cell Xtreme Innovative Dual-Modality Imaging Solution

· Cell Xtreme Dual-Modality Imaging is equipped with advanced functionalities, featuring real-time preview, extended FOV, full-time tracking, and event-driven imaging.

· Cell Xtreme allows you to customize activation rules for multimodal imaging with MI-ODT and MI-SIM, at each stage, and in every loop. Use the Online Image Analysis (OIA) Platform to detect events in one module to drive imaging in the other.

· Online Image Analysis (OIA) monitor: MI-ODT event-driven activates MI-SIM imaging.

· Online Image Analysis (OIA) monitor: MI-SIM event-driven activates MI-ODT imaging.

Applications

1. MI-ODT multi-site imaging of U2OS cell life cycle (e.g. division & death)

2. Cell Xtreme dual-modality imaging verifies the accuracy of mitochondrial segmentation

U2OS cell; Red: mitochondria

CSR Biotech website